SP-Sephadex equilibrium chromatography of bradykinin and related peptides: application to trypsin-treated human plasma.
نویسندگان
چکیده
1 The U. S. Government' s right to retain a nonexclusive royalty-free license in and to copyright covering this paper is acknowledged. , A preliminary re port of part of these findings was presented at the Conference on the Chemistry and Biology of the Kallikrein-Kinin System in Health and Disease. Reston. Va .. Oetober 1974. The abstract is given in Ref. (I). " Visiting Biochemist at Brookhaven National Laboratory, 1970-1972. Supported by Funda~äo de Amparo a Pesquisa do Estado de Säo Paulo (FAPESP). Permanent address: Department of Biochemistry, Escola Paulista de Medicina, Rua Botucatu, R62. Caixa Postal 20.372. 04023 Säo Paulo, Brazil. 4 Visiting Pharmacologist at Brookhaven National Lahoratory. 1973. Supported by Funda<;äo de Amparo a Pesquisa do Estado de Säo Paulo (FAPESPl. Permanent address: Department of Physiological Seiences. Faculty of Dentistry and Pharmacy of Ribeiräo Preto, University 01' Solo Paulo. 14.100 Ribeiräo Preto. Silo Paulo, Brazil. "Visiting Biochemist at Brookhaven National Laboratory, 1972-1974. Supported by Deutsche Forschungsgemeinschaft. Permanent address: Institüt für Klinische Chemie und Klinische Biochemie der Universität München, D-9 München 2. Nussbaumstrasse 20, Germany. \l Visiting Biochemist at Brookhaven National Laboratory. 1970-1972. Supported hy Funda<;äo de Amparo i\ Pesquisa do Estado de Säo Paulo (F APESP). Permanent address: Department of Pharmacology, Faculty of Medicine of Ribeiräo Preto. University of Säo Paulo. 14.100 Ribeiräo Preto, Säo Paulo. Brazil. ; To whom requests for reprints should be made. Biochemist, Biology Department. Brookhaven National Laboratory . and Visiting Professor, Department of Hiochemistry. Faculty of Medicine of Ribeirao Preto. University of Sao Paulo. Permanent address: Department of Biochemistry, Faculty of Medicine of Ribeiriio Preto. University 01' Säo Paulo. 14.100 Ribeiräo Preto, Silo Paulo. Brazil.
منابع مشابه
Immunoglobulin A Preparation from Human Pooled Plasma Using Plasma Fractionation and Ion Exchange Chromatography
Background: In this study we have prepared the IgA solution from normal human plasma using plasma fractionation and ion exchange chromatography. Materials and Methods: Using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction III was prepared as a suitable source for IgA preparation. Then it was treated with caprylic acid for separation of impurities. For enrichm...
متن کاملIsolation and identification of two neutral thyrotropin releasing hormone-like peptides, pyroglutamylphenylalanineproline amide and pyroglutamylglutamineproline amide, from human seminal fluid.
Two tripeptide amides with stuctures similar to thyrotropin releasing hormone were isolated from human seminal fluid and their amino acid sequences determined. The peptides were purified by gel exclusion from Sephadex G50 and were detected by radioimmunoassay with thyrotropin releasing hormone antibody; in addition, N-terminally extended forms were demonstrated by radioimmunoassay after trypsin...
متن کاملIncreased levels of new spasmogenic substances released by trypsin from plasma of hypertensive rats.
The present study was undertaken to evaluate the participation of the kallikrein-kinin system in the normalization of blood pressure after release of the clip in one-kidney, one clip hypertensive rats ( 1K1C ). Kininogen was determined before and after unclipping by tryptic digestion of denatured rat plasma, and spasmogenic activity was measured with isolated guinea pig ileum. In contrast to hu...
متن کاملPartial purification of plasma thromboplastin antecedent (factor XI) and its activation by trypsin.
A persistent puzzle in our understanding of hemostasis has been the absence of hemorrhagic symptoms in the majority of patients with Hageman trait, the hereditary deficiency of Hageman factor (factor XII). One proposed hypothesis is that alternative mechanisms exist in blood through which plasma thromboplastin antecedent (PTA, factor XI) can become active in the absence of Hageman factor. In or...
متن کاملBradykinin-Potentiating Factors of Venom from Iranian Medically Important Scorpions
The venom of animals, including snakes, scorpions, and spiders is a complex combination of proteins, peptides, and other biomolecules as well as some minerals. Among the biomolecules of some animal’s venom, small peptides that lack disulfide bands known as Non-Disulfide Bridge Peptides (NDBPs) potentiate the bradykinin by preventing the conversion of angiotensin 1 to angiotensin 2 using the mec...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Analytical biochemistry
دوره 81 2 شماره
صفحات -
تاریخ انتشار 1977